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CK96
Real-Time PCR System
CK96 is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches international advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.
Up to 6 fluorescence detection channels allowing multiplex PCR
――Simultaneous detection of 5 target genes in 96 samples
FAM,JOE,VIC,HEX,NED,TAMRA and ROX are trademarks of Life
Technologies Corporation and its subsidiaries. VIC and Texas Red
are registered trademarks of Life Technologies Corporation and
its subsidiaries. Cy5 is a registered trademark of GE Healthcare
Bio-Sciences Corp. Quasar are registered trademarks of Biosearch
Technologies, Inc.
Simultaneous scanning of the six-channel shows that
the standard deviation of the Ct value of the FAM
channel is <0.07. No fluorescence signal in other
channels.
Technical Innovation 1
――Effectively reduce multi-color crosstalk and edge effect, no ROX correction required
The multi-color crosstalk caused by the small sample spacing of 96 or 384-well plates has a great influence on the accuracy of
the experimental results, especially in multiplex qPCR detection.
The new optical signal detection system and unique time-resolved scanning can reduce non-target sample optical signal
collection. Thereby high repeatability of single fluorescent channel can be ensured.
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Four different target genes (2 repeats) of FAM/HEX/ROX/Cy5 were simultaneously detected in one reaction tube, and the results
showed that there was almost no cross-interference between the different channels.
New optical scanning detection system――High sensitivity /resolution
• LED light source Efficient and maintenance-free
• Fresnel Lens It greatly reduces the light collection of the non
target area. And the relative position of the detector to the
block hole ensures that one optical detection channel is
aligned with one target to be tested at the bottom.
Different concentrations of plasmids were amplified by
probe assay (concentration from left to right is 5 μg, 500
ng, 50 ng, 20 ng, 10 ng, 5 ng, 500 pg, 50 pg, 5 pg), three
replicates for per concentration. The Ct values difference of
the 10-fold dilution is exactly 3.3. The Ct values difference
of the 2-fold dilution is exactly 1.
Innovative scanning method and time-resolved signal separation technology――High accuracy
• Unique time-resolved scanning method
The different fluorescence signals of the same sample are
collected at different times.
The high-speed stepping motor and the highly sensitive
detector ensure that all signal acquisition of the entire
sample plate is completed in a short time.
Multi-channel scanning for probe assay
• Innovative detection channel arrangement Interlaced
arrangement of upper and lower channels further
reduces inter-hole and multi-color fluorescence crosstalk.
• Double FAM scanning for melting curve Scan time is
shortened a lot.
Double FAM scanning for melting curve
Technical Innovation 2
――Unique edge temperature compensation technology
• Both temperature accuracy and uniformity are ±0.2℃
• Module maximum ramp rate is 6 ℃ / sec
• The average sample ramp rate is 2.2° C / sec
• With a unique outframe protection design, the unit can achieve very even
temperature across the whole plate. Effectively reduce the edge temperature variance.
The Tm values of 96 replicate wells were detected. The results showed that the temperature uniformity difference was
＜±0.2℃@81℃.
The melting curves of 48 replicate samples
Technical Parameters
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