UFluo ONE I / UFluo ONE II / UFluo ONE III
UFluo ONE Fluorometer
UFluo ONE Fluorometer
Fast and sensitive quantification of DNA, RNA, and protein
UFluo One Fluorometer is a benchtop fluorometer designed for highly accurate quantification of DNA, RNA, micro RNA, and protein. It is a dual-channel fluorometer and is ideal for the measurement of scarce and difficult-processing sample. The fluorometer has a 7 inch touch screen and intuitive user interface, making it easy to select and run your desired assay with results displayed in just a few seconds.
Key features
• High sensitivity —more sensitive than UV absorbance–based quantification
• Ideal for precious samples —uses as little as 0.5 μL of sample
• Flexible data management —store up to 100,000 samples internally or export results to the USB drive, or computer
• Portable with small footprint —small enough to fit in any lab
• Open reagent system — users can choose reagents freely, sample tube can be replaced, can order 0.5ml/0.2ml PCR tube
• Intuitive touchscreen —quickly get to data generation
• Accuracy and speed —accurately quantifies DNA, RNA, or protein in less than 3 seconds per sample
|
Model
|
Channel
|
Excitation wavelength |
Emission wavelength |
|
UFluo ONE I
|
Blue |
470nm |
525nm |
|
Red |
625nm |
690nm |
|
|
UFluo ONE II |
Blue |
470nm |
525nm |
|
Green |
525nm |
620nm |
|
|
UFluo ONE III
|
Blue |
470nm |
525nm |
|
UV |
365nm |
460nm |
Common reagents and application
|
Excitation wavelength |
Commonly used fluorescent reagents |
Applications |
|
365 |
Hoechst33258, 4-MU,EnZCheK Caspase |
Plant GUS report gene detection, cell apoptosis detection |
|
470 |
PicoGreen®, oligreen，RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™ |
DsDNA,ssDNA quantitative, green fluorescent protein (GFP), fluorescent detection, protein quantitative |
|
525 |
Rhodamine, Cy3, RFP Vybrant Cytotoxicity |
Rhodamine detection, Cy-3 fluorescence labeling detection, red fluorescent protein (RFP) gene detection, cell activity toxicity detection |
|
625 |
Cy5, Quant-iT RNA |
Cy-5 fluorescence labeling detection, RNA quantitative |
Specifications
|
Physical characteristics |
Instrument type |
Benchtop fluorometer |
|
Dimensions(W*D*H) |
(26cm× 16 cm× 6 cm); rectangular shape |
|
|
|
Weight |
1.0 kg |
|
|
Operating Power |
DC 9V 1.5A |
|
Technical |
Dynamic range |
5 orders of magnitude |
|
|
Detectors |
Photodiodes |
|
|
Repeatability |
<1.5% |
|
|
Stability |
<1.5% |
|
|
Linearity |
R2>0.995 |
|
|
Sensitivity |
dsDNA: 0.5ng/ml |
|
|
Tube type |
0.2mL/0.5mL |
|
Hardware |
Display |
7-inch capacitive touchscreen with high resolution color display |
|
|
Output ports |
3× USB ports |
|
|
Networking capability |
Connection via the PC USB port using an Ethernet cable |
|
|
Power supply |
AC adaptor with power cords |
|
USB drive |
Capacity |
16 Gigabyte |
